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The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development

The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and ar...

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Tác giả chính:	Mohammad, H Dezfulian, Danielle, M Soulliere, Rajdeep, K Dhaliwal
Ngôn ngữ:	English
Năm xuất bản:	Public Library of Science (PLoS) 2018
Chủ đề:	Medicine Science
Truy cập Trực tuyến:	http://lrc.quangbinhuni.edu.vn:8181/dspace/handle/DHQB_123456789/4036
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spelling	oai:localhost:DHQB_123456789-40362018-10-22T08:44:18Z The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development Mohammad, H Dezfulian Danielle, M Soulliere Rajdeep, K Dhaliwal Medicine Science The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. Phylogenetic analysis based on deduced polypeptide sequence organizes the family of ASK proteins into 7 clades. The complexity of the ASK gene family, together with the close structural similarity among its members raises the prospect of significant functional redundancy among select paralogs. We have assessed the potential for functional redundancy within the ASK gene family by analyzing an expanded set of criteria that define redundancy with higher resolution. The criteria used include quantitative expression of locus-specific transcripts using qRT-PCR, assessment of the sub-cellular localization of individual ASK:YFP auto-fluorescent fusion proteins expressed in vivo as well as the in planta assessment of individual ASK-F-Box protein interactions using bimolecular fluorescent complementation techniques in combination with confocal imagery in live cells. The results indicate significant functional divergence of steady state transcript abundance and protein-protein interaction specificity involving ASK proteins in a pattern that is poorly predicted by sequence-based phylogeny. The information emerging from this and related studies will prove important for defining the functional intersection of expression, localization and gene product interaction that better predicts the formation of discrete SCF complexes, as a prelude to investigating their molecular mode of action. 2018-09-10T03:00:10Z 2018-09-10T03:00:10Z 2012-11 1932-6203 (Online) http://lrc.quangbinhuni.edu.vn:8181/dspace/handle/DHQB_123456789/4036 en Public Library of Science (PLoS)
institution	Trung tâm Học liệu Đại học Quảng Bình (Dspace)
collection	Trung tâm Học liệu Đại học Quảng Bình (Dspace)
language	English
topic	Medicine Science
spellingShingle	Medicine Science Mohammad, H Dezfulian Danielle, M Soulliere Rajdeep, K Dhaliwal The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
description	The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. Phylogenetic analysis based on deduced polypeptide sequence organizes the family of ASK proteins into 7 clades. The complexity of the ASK gene family, together with the close structural similarity among its members raises the prospect of significant functional redundancy among select paralogs. We have assessed the potential for functional redundancy within the ASK gene family by analyzing an expanded set of criteria that define redundancy with higher resolution. The criteria used include quantitative expression of locus-specific transcripts using qRT-PCR, assessment of the sub-cellular localization of individual ASK:YFP auto-fluorescent fusion proteins expressed in vivo as well as the in planta assessment of individual ASK-F-Box protein interactions using bimolecular fluorescent complementation techniques in combination with confocal imagery in live cells. The results indicate significant functional divergence of steady state transcript abundance and protein-protein interaction specificity involving ASK proteins in a pattern that is poorly predicted by sequence-based phylogeny. The information emerging from this and related studies will prove important for defining the functional intersection of expression, localization and gene product interaction that better predicts the formation of discrete SCF complexes, as a prelude to investigating their molecular mode of action.
author	Mohammad, H Dezfulian Danielle, M Soulliere Rajdeep, K Dhaliwal
author_facet	Mohammad, H Dezfulian Danielle, M Soulliere Rajdeep, K Dhaliwal
author_sort	Mohammad, H Dezfulian
title	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
title_short	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
title_full	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
title_fullStr	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
title_full_unstemmed	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
title_sort	skp1-like gene family of arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development
publisher	Public Library of Science (PLoS)
publishDate	2018
url	http://lrc.quangbinhuni.edu.vn:8181/dspace/handle/DHQB_123456789/4036
_version_	1717292468320337920
score	9,463379

The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development

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